Liston Group

Liston Group
Liston Group

Research Summary

The Liston laboratory works on regulatory T cells. These are a type of white blood cell that act to suppress the rest of the immune response, preventing spontaneous autoimmune disease and acting as a rheostat to control just how active our immune system is. The number of these cells in our blood goes up as we get old, which may contribute to the immune-suppressed state of older persons. We seek to understand these cells, using both patient material and mouse models, so that we can harness their power to fine-tune the immune system for healthy ageing.
 

Latest Publications

Willemsen M, Staels F, Gerbaux M, Neumann J, Schrijvers R, Meyts I, Humblet-Baron S, Liston A Immunology

Inborn errors of immunity are a heterogenous group of monogenic immunological disorders caused by mutations in genes with critical roles in the development, maintenance or function of the immune system. The genetic basis is frequently a mutation in a gene with restricted expression and/or function in immune cells, leading to an immune disorder. Several classes of inborn errors of immunity, however, result from mutation in genes that are ubiquitously expressed. Despite the genes participating in cellular processes conserved between cell types, immune cells are disproportionally affected, leading to inborn errors of immunity. Mutations in DNA replication, DNA repair or DNA damage response factors can result in monogenic human disease, some of which are classified as inborn error of immunity. Genetic defects in the DNA repair machinery are a well-known cause of TBNK severe combined immunodeficiency. An emerging class of inborn errors of immunity is those caused by mutations in DNA replication factors. Considerable heterogeneity exists within the DNA replication-associated inborn errors of immunity, with diverse immunological defects and clinical manifestations observed. These differences are suggestive for differential sensitivity of certain leukocyte subsets to deficiencies in specific DNA replication factors. Here, we provide an overview of DNA replication-associated inborn errors of immunity and discuss the emerging mechanistic insights that can explain the observed immunological heterogeneity.

+view abstract The Journal of allergy and clinical immunology, PMID: 36395985 14 Nov 2022

Whyte CE, Tumes DJ, Liston A, Burton OT Immunology

Recent advances in flow cytometry have allowed high-dimensional characterization of biological phenomena, enabling breakthroughs in a multitude of fields. Despite the appreciation of the unique properties of antigens and fluorophores in high-parameter panel design, staining conditions are often standardized for short surface stains, regardless of antibody affinity or antigen accessibility. Here, we demonstrate how increasing antibody incubation times can lead to substantial improvements in sensitivity, maintaining specificity, and reducing background, while also significantly reducing the costs of high-parameter cytometry panels. Furthermore, overnight staining reduces the influence of interexperimental variability, assisting accurate pooling over experiments over extended time courses. We provide guidance on how to optimize staining conditions for diverse antigens, including how different fixation strategies can affect epitope accessibility. Overnight staining can thus substantially improve the resolution, repeatability, and cost-effectiveness of high-parameter cytometry. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.

+view abstract Current protocols, PMID: 36373983 Nov 2022

Liston A Immunology

No abstract available

+view abstract Immunology and cell biology, PMID: 36317807 01 Nov 2022

Group Members

Adrian Liston

Group Leader

Magda Ali

PhD Student

Meryem Aloulou

Visiting Scientist

Ronya Assady

Visiting Student

Pascal Bielefeld

Visiting Scientist

Orian Bricard

Visiting Scientist

Oliver Burton

Senior Postdoctoral Scientist

Francine Chenou

Visiting Scientist

Amy Dashwood

PhD Student

James Dooley

Senior Staff Scientist

Vaclav Gergelits

Postdoc Research Scientist

Keith Harrison

Visiting Student

Lubna Kouser

Postdoc Research Scientist

Miroslav Kratochvil

Visiting Scientist

Ntombizodwa Makuyana

PhD Student

Alena Moudra

Postdoc Research Scientist

Omar Shabana

Visiting Student

Samar Tareen

Visiting Scientist

Emily Thomas

Visiting Student

Daria Vdovenko

Visiting Scientist

Rafael Valente Veiga

Postdoc Research Scientist