4 lasers (488nm, 355nm, UV, 405nm, 635nm), able to detect 10 colours.
The LSRII has been part of the core facility since 2006 and is a much-loved machine.
Equipped with a UV laser, it allows scientists to look at Calcium Flux, DAPI/Hoechst cell cycle as well as UV excited fluorochromes.
Propel Labs YETI analyser allows users to take their analysis to another dimension. Not only is it a 5 laser, 30 parameter machine but it can be used for high-throughput analysis using tubes or plates. This machine allows simultaneous detection of 27 fluorescent and 3 scatter parameters (FSC and SSC from 488nm laser plus FSC from 405nm laser).
The filter configuration enables users to utilise a range of commercially available fluorochromes, allowing multi-colour phenotyping above the Fortessa’s capabilities whilst also giving the user more flexibility of dye combinations. The machine can be used in ‘stat’ mode to run individual tubes, but can easily be used with racks of 5ml and Eppendorf tubes to allow easy of use and walk away analysis.
Analysis of 96 well plates can be done in around 15 minutes making it perfect not only for in depth phenotyping studies but also screening. There is also a 384 well plate capability.
4 laser (488nm, 405nm, 561nm, 640nm). This imaging cytometer produces multiple high resolution images of every cell directly in flow, giving quantitative information about each cell in up to 12 channels simultaneously.
The Imagestream is an image cytometer that allows quantitative data to be acquired as well as images of every event passing through the instrument to be recorded. This machine can analyse up to 5000 cells/sec. This clever machine allows the user to look at features of cells such as co-localisation, staining location, cell-cell location, spot counting and cell death analysis.
Members of the core facility are available to help set up and optimise assays and help with post-acquisition analysis of the data.