BuildingCyt Flow Cytometry Courses

Training course 2018

We would like to welcome delegates back to face to face courses.

The courses are hosted by experts in flow cytometry using interactive tools to aid learning and to give immediate feedback to delegates.

A 10% discount on the face to face courses is given to scientists of companies based on the Babraham Research Campus or a company that uses Babraham Institute Flow Cytometry Core Facility (code to use is 10offBRC)

We are planning on running face to face courses at the Babraham Institute in the 2023 as well as continuing with virtual courses. The content of the courses will be the same, however the face to face courses have the added benefit of liaising with the core tutors during breaks, networking with delegates. Refreshments will be included for the in person courses including lunch, morning and afternoon tea/coffee. In person Module 2 will also include a tour of the BI flow cytometry facility, with demos on cell sorting, flow analysis, image cytometry and Full Spectral Cytometry.


In person @ Babraham Institute, Babraham Research Campus, Cambridge (9am - 5pm)

Module 1 – One day Fundamentals of Cytometry Course

  • 22nd February 2023
  • 14th March 2023
  • 4th May 2023
  • 8th June 2023


Module 2- Two Day Beyond the Basics course

  • 27th & 28th April 2023
  • 29th & 30th June 2023


FlowJo analysis course - 3 hours

  • Dates to be announced soon


Virtual – interactive course using Zoom (9.30-5pm

Module 1 – One day Fundamentals of Cytometry Course

  • New dates for 2023 coming soon


Module 2- Two Day Beyond the Basics course

  • 29th and 30th November 2022


FlowJo analysis course - 3 hours

  • New dates for 2023 coming soon


Please note these are live interactive courses and will not be recorded. Delegates will be sent a Zoom link and course notes to download before the course.


To Book please visit our Eventbrite pages


Description of the courses:

Module 1: Fundamentals of Flow Cytometry

This module is aimed at those new to flow cytometry and those wanting to understand the basics of the technology before attending Module 2.  This can be taken as a standalone one day course or can be taken as a building block for the next module.   The course starts with an introduction to this quickly evolving technology, its importance and resources that are available.

One of the main aims of this course is to give the delegate a firm understanding of fluorescence, fluorochromes and the workings of a cytometer.  This is followed by talks on fluorescence compensation, sample preparation, basics of experimental design, data analysis and reporting your flow cytometry data.  

It is highly recommended that all delegates attend Module 1 before attending Module 2, even those with years of experience in flow cytometry.  This is to ensure that all participants get the most out of the modules and to confirm that the basics of flow cytometry have been grasped before tackling more advanced topics in Module 2. 

It is highly recommended that delegates should attend Module 1 before attending Module 2 as these are linked modules.


Module 2: Beyond the Basics

The follow up two day module takes the knowledge from Module 1 and builds on it to give the delegates confidence in designing, running, analysing and presenting their flow cytometry data. Following an introduction to the module, the delegates will hear about cytometer performance including optimisation and troubleshooting.

There are sessions on multicolour flow cytometry giving delegates invaluable experience in multi-colour compensation and experimental planning including panel design.  The varied applications of flow cytometry are explained including cell sorting, apoptosis and functional studies.  Delegates are taken through experiments, step by step including, planning, executing, analysing and presenting data.


FlowJo Analysis course

New to Autumn 2022, please join our resident FlowJo expert, Christopher Hall (Deputy Manager, BI Flow Cytometry Core) who will take you through the basics and advanced use of FlowJo Post-Acquition software.  You will need to download FlowJo onto your Laptop (and bring it for the in person courses). The courses use pre-prepared files from FlowJo so no FlowJo licence is required.

Course includes:

  • -Starting from scratch - the components of FlowJo software, uploading data, making groups, keywords
  • Compensation, manual and automatic inc. Autospill
  • Gating, back gating and using layout tools
  • Batching samples.
  • Making presentation ready figures, tables and overlays
  • Saving templates, worksheets and experiments
  • Advanced topics including cell cycle analysis, proliferation, T-SNE and other clustering methods

There will also be plenty of opportunity to ask Chris questions, and it will be possible for him to help with your specific FlowJo analysis questions.

More information about the content of the course can be found here

Testimonials from recent courses include:

Module 1

‘One of the best technical training courses I have attended in my scientific career’

‘The course provides a good grounding in flow cytometry that will facilitate high quality data generation and greater understanding of results.‘

‘The course gave me an excellent understanding of the basics of flow cytometry. I now feel confident in designing, running and analysing my own experiments.’

‘Would recommend to get the flow started :)’

‘It was a good course that covered very well the basics of flow cytometry. The course leads are very knowledgeable and made it easy to follow and understand what was being presented.‘

‘Flow cytometry principles and best practices were presented in a user-friendly yet rigorous way. I got really engaged and I can't wait to apply what I have learnt to my experiments. Being online made the course accessible also by people living in other countries, like me’

Module 2

‘I thought the course was informative and thought provoking. The use of polls and practical exercises kept the audience engaged and focused personal understanding of aspects of experimental design.’

‘Excellent course going into the details of how to do compensation, prepare samples and plan a panel design for multi-colour flow cytometry experiments. Very interactive course with open and friendly leaders who answered any queries related or not to the slides throughout the course.’

‘I highly recommend this course to anyone interested in flow cytometry, from total beginners to advanced users. No matter your level you will definitely take away a lot of useful information. The course material is really well-designed and covers most aspects of the technique, from the theory behind it to the best practices in experimental design, running an experiment, troubleshooting and analysing your data. The instructors are very knowledgeable and really good at explaining - it is obvious they have put a lot of work into designing this course.’

‘This course is so informative and perfect for beginners. The instructors were so kind and knowledgeable and were happy and able to answer all our questions. I felt very included and cared for as if we were attending an in-person course despite it being online format. I am thankful it was online as I would not have been able to attend without taking a plane ride over the Atlantic! :) Would highly recommend it to anyone new to flow!’

‘Excellent course giving an overview of flow cytometry that I highly recommend - presentations on zoom were engaging and teachers were excellent.’


Terms and Conditions of  Flow Cytometry Courses run by Babraham Institute Enterprise Ltd (BIE)

  • No refunds, transfers or deferrals can be made if less than 14 days’ notice is given.

  • BIE reserves the right to cancel, reschedule or change the location or content of a course without penalty if considered necessary.

  • BIE undertakes to inform customers as soon as the change is made and customers may book an alternative course at no extra charge, or receive a full refund or credit against future courses.

  • If a customer wishes to cancel a course, provided more than 14 days’ notice prior to the course start date is given, the tuition fee will be refunded less a £50 cancellation fee.

  • If a customer wishes to transfer to another course, provided more than 14 days’ notice prior to the course start date is given and subject to availability, no additional charge will be made, other than for any new study materials that may be required.

  • It may be possible to transfer your place on a course to another member of your organisation, but this will be assessed by BIE on a case to case basis.