A new mechanism that affects how our immune cells perform – and hence their ability to prevent disease – has been discovered by an international team of researchers led by Cambridge scientists. The Babraham Institute made fundamental contributions to this research through the use of its cutting-edge lipidomics facility and expertise.
To date, researchers have identified hundreds of genetic variants that increase or decrease the risk of developing diseases from cancer and diabetes to tuberculosis and mental health disorders. However, for the majority of such genes, scientists do not yet know how the variants contribute to disease – indeed, scientists do not even understand how many of the genes function.
One such gene is C13orf31, found on chromosome 13. Scientists have previously shown that variants of the gene in which a single nucleotide – the A, C, G and T of DNA – differs are associated with risk for the infectious disease leprosy, and for two chronic inflammatory diseases – Crohn’s disease and a form of childhood arthritis known as systemic juvenile idiopathic arthritis.
In a study published today in the journal Nature Immunology and led by the University of Cambridge, researchers have studied how this gene works and have identified a new mechanism that drives energy metabolism in our immune cells. Immune cells help fight infection, but in some cases attack our own bodies, causing inflammatory disease.
Using mice in which the mouse equivalent of the C13orf31 gene had been altered, the team showed that the gene produces a protein that acts as a central regulator of the core metabolic functions in a specialist immune cell known as a macrophage (Greek for ‘big eater’). These cells are so named for their ability to ‘eat’ invading organisms, breaking them down and preventing the infection to spread. The protein, which the researchers named FAMIN (Fatty Acid Metabolic Immune Nexus), determines how much energy is available to the macrophages.
The researchers used a gene-editing tool known as CRISPR/Cas9, which acts like a biological ‘cut and paste’ tool, to edit single nucleotides in the C13orf31 gene in the mouse genome and produced mice with altered versions of the FAMIN protein. This genetic edit made the mice more susceptible to sepsis (blood poisoning) and demonstrated that even a tiny change to our genetic makeup could have a profound effect. Analysing the metabolic pathways active in macrophages from normal and genetically altered mice showed that FAMIN is an important modulator of metabolic activity. Aberrations in this mechanism decreases the cell’s ability to perform its normal function, controlling its capacity to kill bacteria and release molecules known as ‘mediators’ that trigger an inflammatory response, a key part of fighting infection and repairing damage in the body.
Professor Arthur Kaser from the Department of Medicine at the University of Cambridge, who led the research, said: “By taking a disease risk gene whose role was completely unknown and studying its function down to the level of a single nucleotide, we’ve discovered an entirely new and important mechanism that affects our immune system’s ability to carry out its role as the body’s defence mechanism.
“Although it’s too early to say how this discovery might influence new treatments, genetics can provide invaluable insights that might help in identifying potential drug targets for so-called precision medicines, tailored to an individual’s genetic make-up.”
Professor Michael Wakelam, Institute Director and group leader at the Babraham Institute, who oversaw the lipidomics analysis, said: “This project brought together the expertise of many groups in Cambridge emphasising the increasing importance of a multidisciplinary approach to understand the biology underpinning the body’s defence mechanisms. The Institute’s lipidomics capability provides a unique opportunity to diagnose metabolic changes in health and disease.”
The research was largely funded by the European Research Council and the Wellcome Trust, with support from National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre. The Babraham Institute is strategically funded by the Biotechnology and Biological Sciences Research Council.
Peritoneal macrophages fluorescently stained for proteins important in cell motility: vinculin (red) and actin (green). Image by Manuel Diaz-Munoz.
Cader et al. C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nature Immunology; 1 Aug 2016; DOI:10.1038/ni.3532
Qifeng Zhang, (then) Head of the Lipidomics facility
Michael Wakelam, Institute Director and Signalling programme group leader
Mice were bred and maintained in specific pathogen-free conditions at the Central Biomedical Services (CBS) facility, University of Cambridge and at the Wellcome Trust Sanger Institute. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted and conducted with approval of the UK home office.
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01 August 2016