A change of heart

A change of heart

A change of heart

The heart is an amazingly adaptable organ, responding to the needs of the organism throughout life, such as through periods of increased demand by pumping harder, faster, and also growing to accommodate longer-term requirements such as that experienced in pregnancy or as a response to intense exercise.
Some cardiac diseases, such as prolonged high blood pressure and heart attacks, also cause an increase in the heart’s muscle mass but dangerously this results in a reduction in cardiac output and can cause an irregular heart rhythm. This growth is called pathological cardiac hypertrophy and eventually leads to heart failure and death. Cardiovascular diseases account for a third of all deaths in the UK.
Now, researchers at the Babraham Institute, UK, University of Leuven, Belgium, University of Oslo, Norway and Karolinska Institute, Sweden, have uncovered the molecular control mechanisms responsible for the different biological changes seen in cardiac hypertrophy induced by pathology compared to exercise. These findings point the way for the design of new treatments for heart disease.
Their research, published in the Journal of Clinical Investigation, compared the differences between hypertrophic heart growth in rats as a result of exercise – which is beneficial – and heart growth induced by pathology – in this case, increased load. Specifically, they compared epigenetic marks responsible for locking cells in their final developed state – important for preventing cells from switching to a less differentiated state. Notably for their analysis, the researchers employed a powerful cell sorting technique to allow them to study pure populations of heart muscle cells (cardiomyocytes) rather than a mix of all cell types in the heart – which, due to an alteration in composition during disease, would confound analysis. 
They found a mechanism explaining how, in the case of pathological cardiac hypertrophy, cardiomyocytes lose their adult cellular state and regress back towards their foetal form, switching on genes that were originally expressed as the heart develops in the embryo and usually permanently switched off after birth.
Professor Wolf Reik, Head of the Epigenetics Programme at the Babraham Institute, said: “We found that a very important repressive methylation mark is lost by cells in cardiac hypertrophy. The function of this mark is to lock adult cardiomyocytes in their adult state. The loss of the mark leads to inappropriate gene expression as shown by the re-expression of genes usually only seen late in embryo development.”
The research also analysed human cardiomyocytes and importantly the same molecular changes were seen, demonstrating that the same epigenetic factors underlie cardiac hypertrophy and disease remodelling in humans.
Professor Llewelyn Roderick, former group leader at the Babraham Institute, now Professor in the Department of Cardiovascular Sciences at KU Leuven, commented: “Our research has defined a novel epigenetic-based mechanism which explains the contrasting outcomes of cardiac remodelling caused by exercise and pathology. By identifying the epigenetic determinants and the responsible epigenetic enzymes controlling these different forms of cardiac myocyte hypertrophy, as well as how the epigenetic modifiers are themselves regulated by micoRNAs, we provide a potential strategy for epigenetic therapy for adverse cardiac remodelling. This work highlights the value of collaborative research to allow analysis from physiology to molecule and back again.”
This work was funded by the BBSRC which provides strategic support to the Babraham Institute, The Royal Society and an Odysseus award from the Research Foundation Flanders FWO to support the aspects of this work undertaken at the Babraham Institute. The collaborative work at the University of Oslo was supported by the KG Jebsen Cardiac Research Center and the Center for Heart Failure Research of the University of Oslo and by the Anders Jahres Fund for the Promotion of Science. At the Karolinska Institute, the work was supported by the Swedish Research Council, the Ragnar Söderberg Foundation, the Jeansson Foundations, and the Åke Wibergs foundation.

Image description:

Microscopic image of a piece of rat heart tissue in which the proteins involved in contraction are stained in white, the cell membranes in yellow and nuclei in blue. The nuclei that are from cardiomyocytes and not the other cells of the heart have red outlines. Credit: Hanneke Okkenhaug, Babraham Institute and Llewelyn Roderick, KU Leuven.

Publication reference:

Thienpont, Aronsen, Robinson et al. (2016) The H3K9 dimethyltransferases Ehmt1/2 protect against pathological cardiac hypertrophy. Journal of Clinical Investigation

Affiliated authors (in author order):

Bernard Thienpont, former postdoc and Marie Curie Fellow (Synergy grant funding, Roderick group) at the Babraham Institute, now postdoctoral scientist at VIB Vesalius Research Center and KU Leuven
Emma Robinson, former PhD student (Roderick lab), Epigenetics Programme and Wellcome Trust Cardiovascular & Metabolic Disease PhD programme
Hanneke Okkenhaug, Deputy Manager, Imaging Facility (previously Roderick group)
Elena Loche (previously Da Vinci Student, Roderick Group)
Arianna Ferrini, former Da Vinci student, (Roderick group)
Patrick Brien, former PhD student (Roderick group)
Asmita Agrawal, former PhD student (Roderick group)
Wolf Reik, Head of Epigenetics programme and group leader at the Babraham Institute, and associate faculty at the Wellcome Trust Sanger Institute
Llewelyn Roderick, former group leader at the Babraham Institute, now Professor in the Department of Cardiovascular Sciences at KU Leuven

Animal research statement:

As a publicly funded research institute, the Babraham Institute is committed to engagement and transparency in all aspects of its research. The research presented here used rats in which either exercised-induced or pathological hypertrophy was developed. After six weeks of either treatment, the rats were humanely killed and their hearts were removed for cellular and genomic analysis.  Mice were also used in this research. Mice were implanted with osmotic minipumps or received intravenous (jugular vein) injection or underwent surgery. Irrespective of which procedure was used, all mice were humanely killed two weeks later to study the effects of the different procedures. 
Experiments involving animals were in accordance with the Animals Act 1986 (UK), and with Regulations on Animal Experimentation under The Norwegian Animal Welfare Act, approved by the Norwegian Animal Research Authority (FDU application 3301, 3820 and 5338).
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