Lipidomics

The Lipidomics facility uses chromatographic techniques coupled to tandem mass-spectrometry instruments to study a wide range of chemical species including neutral, phospho-and sphingolipids and aims to provide a detailed understanding of the lipid roles in cellular structures, signalling pathways and regulation in health and disease.

Key services

Identification and quantification of biologically significant lipids from mammalian cells tissue, C. elegans, yeast, and immunoprecipitated protein complexes. If you require the analysis of any other matrix, please don’t hesitate to contact us. Instrumentation and the current developed methods allow for the analysis of up to 9 classes of neutral lipids, 14 classes of phospholipids and 9 classes of sphingolipids. We adapt and establish methodologies to analyse lipid proposed by collaborators (internal and external of BI) together with any other chemicals of biological significance. Please contact us if you are interested in discussing your project and start a collaboration.

Expertise and Advice

Our team offers a multidisciplinary platform for the analysis of lipids in biological systems. The Lipidomics Facility scientists possess expertise in mass spectrometry, chromatography methods applied to chemical and biological questions. A collaboration with our facility will yield a powerful analysis of the lipids contained in your experimental samples, furthermore, we will provide a in-depth careful statistical analysis of the data using R scripts developed in collaboration with the bioinformatics group on-site. We interpret the results and bioinformatic pathway analysis is performed for the determination of lipid metabolism enzymes activities modified by treatment or physiological state, providing as well as graphical representations of the results of the experiment.

Key Facts

Over 15 scientific collaborations, both local and international, in a variety of biological science fields; 8 novel quantitative methods developed in the facility to offer a comprehensive analysis to cover the cellular lipidome; Over 30 lipid classes can be analysed and quantified with the lipidomics facility resources. Only last year, 295 samples were processed which translates into a total of 1,398 chromatographic runs and 5,457 lipid classes analysed (this data does not includes the molecular species reported for each replica). The analysis included LC-MS run, relative quantitation, statistical analysis and data interpretation.

Facility flier (pdf)

Contact:

lipidomics@babraham.ac.uk

Lipidomics workflow
Lipidomics detail
Lipidomics structure