High content imaging (HCI) is essentially a three-step process: automated image acquisition, automated image processing and data analysis. HCI is used to increase throughput of repetitive imaging operations, and provides data that are less subjective and more quantitative. Automated imaging also opens up the possibility of using images of single cells in high throughput assays such as RNAi/CRISPR screens and drug library screens.
There are two automated imaging systems in the Facility that can be used for HCS applications. The first is an InCell 6000 which is a semi-confocal imaging system designed to image cells cultured in standard format multi-well plates (although it can also image cells on slides). In addition to imaging fixed cells, the system has many features which make it suitable for live cell analysis including temperature control, CO2 regulation and liquid handling. The InCell 6000 is equipped with a Caliper Twister II robotic plate loader which significantly increases the throughput capability of the system. Potential applications for this system include live/dead analysis, cell cycle analysis, apoptosis, cytoplasmic/ nucleus translocation assays, DNA damage, tube formation (angiogenesis), neurite outgrowth, micronucleus assays, morphometric analysis (tubulin) and cell migration.
The second system is a MetaSystems Metafer/MetaCyte. This system is built around a Zeiss Axio Imager Z2 upright microscope with a motorised stage that can hold 8 slides at a time. The system has broad application, but is optimised for cytogenetic analysis including metaphase detection, metaphase relocation and capture (single or multi-colour), interphase detection, metaphase aberration scoring (e.g. breakage/dicentric formation) and micronuclei detection. The system has a module for Fluorescence In Situ Hybridisation (FISH) analysis which will extract information such as spot count (absolute, per cell, or mean per cell), spot distance (including signal fusions/co-localisation), spot distribution patterns (e.g. mixed cell populations) and spot positioning.
Contact us to find out how we can help with your high content imaging requirements.