Confocal Imaging

Confocal Imaging

Confocal microscopy is an optical sectioning technique which removes out-of-focus light by using a pinhole in the emission light path. This creates an image with improved contrast and therefore ability to resolve finer detail.  By acquring a series of optical sections through a specimen it is possible to create a three dimensional model of the sample.

The Imaging Facility has a number of different confocal microscopes including three point-scanning systems and one spinning disk system.  Different experiments require different approaches, but genereally speaking, point-scanning confocal microscopy offers the greatest flexibility whereas spinning disk confocal micoscopy is the preferred option for live cell imaging.

Our Systems:

Carl Zeiss LSM 780 Confocal

Point-scanning confocal
405, 488, 561 & 633 nm excitation
2x standard PMTs, 1x 32 channel spectral GaAsP detector
10x, 20x, 40x, 63x objectives
FCS module

Nikon A1-R

Point-scanning confocal
405, 488, 561, 635 nm excitation
4x standard PMTs
10x, 20x, 40x, 60x objectives

Olympus FV1000

Point-scanning confocal
405, 440, 488, 515, 561 & 633 nm excitation
10x, 20x, 40x, 60x objectives
SIM scanner

Andor Revolution

Spinning disk confocal
405, 440, 488, 515, 561 & 635 nm excitation
Piezo objetive stepper
2x EM-CCD cameras
Full enclosure incubation