EU Life Lecture - The regulatory potential of protein post-translational modifications
"The regulatory potential of protein post-translational modifications in chromatin biology and gene expression investigated by MS-based proteomics"
Chromatin is a highly dynamic, well-organized and yet ill-defined protein-DNA-RNA structure that controls various DNA-dependent processes. A large number of site-specific post-translational modifications of histones (hPTMs) contribute to the maintenance and modulation of chromatin plasticity, gene activation, DNA replication and repair, and a variety of other biological processes and disease states. The observation of the diversity, frequency and co-occurrence of histone modifications at distinct genomic loci led to the notion that these marks create a molecular barcode, read by effector proteins that translate it into a specific transcriptional state, or process, on the underlying DNA. However, the molecular details of its working mechanisms are only partially characterized. More recently, various technological progresses have enabled the detection of these PTMs on an increasing number of non-histone proteins, involved in a variety of biological processes.
Recent achievements made Mass Spectrometry (MS) and quantitative proteomics excellent tools to help understanding how histone and non-histonic PTMs mediate the structural-functional state of chromatin. My team contributed to the field by setting-up distinct MS-proteomics strategies, combined with various biochemical methods of enrichment of chromatin and extra-chromatin proteins, to investigate chromatin plasticity and nuclear dynamics governed by post-translational modifications.
The talk will offer an overview of the MS-proteomics strategies developed to gain insights into chromatin biology, with emphasis on: the proteomic dissection of chromatin regulatory regions; the hPTMs-analysis of clinical specimens and the recent achievements on the methyl-proteome profiling and its impact in DDR and miRNA biogenesis.
Tiziana Bonaldi completed her PhD from the Parco Biomedico San Raffaele (Milano) and two post-doctoral studies, first from the Ludwig Maximillians University (2003-2006) and from the Max Planck Institute of Biochemistry in Munich (2006-2008). During her post-doctoral work she became progressively expert in the field of chromatin biochemistry and chromatin-proteomics, using high-resolution MS to decrypt the code of histone modifications and to dissect how distinct chromatin determinants synergize to enforce specific gene expression patterns. Since 2008 she is Group Leader at the Department of Experimental Oncology of the European Institute of Oncology in Milano, where she is continuing focusing on the use of MS-proteomics to investigate chromatin-mediated gene expression regulation. She has published 43 papers in peer-reviewed international journals and has been serving as an editorial board member of various journals in the field of MS-proteomics. She was awarded the EMBO Long –term fellowship in 2004 for her post-doctoral research, the Armenise-Harvard Career Development Award in 2008 and the Inner-wheel award “Women in Science” in 2010, for her scientific achievements.
EU Life and the Babraham Institute
EU-LIFE is an alliance of European life science research centers that have joined forces to support, collaborate and strengthen scientific research in Europe, working together to achieve advancement and further understanding in Life Sciences.
The growing consortium currently consists of 13 partner Institutes and over 7,000 scientific staff with the Babraham Institute, currently the only UK representative, joining in 2013.
As part of the collaboration, the Babraham Institute are now proud to host a series of lectures to highlight and support the latest research from fellow members.
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