HuR and Posttranscriptional Gene Regulation: Sometimes the Small Fish are More Important
It is now well appreciated that posttranscriptional gene regulation mediated by RNA binding proteins (RBPs) and microRNAs (miRNAs) play important roles in controlling eukaryotic gene expression. RNA immunoprecipitation techniques, such as RIP-Chip and RIP-Seq, can identify novel mRNA targets which may have escaped detection using traditional transcriptomic profiling methods. However, many of these approaches have been performed in cells lines and therefore are limiting in terms of studying primary tissues and disease processes in animals. Our group has developed conditional knock-out models to study the role of HuR in allergic airway inflammation and neuroinflammation. These techniques and models can readily be adapted for use in a variety of disease processes. We will present recent data on the role of HuR in controlling CD4+ T cell differentiation and IL-2 homeostasis. Additionally, we will also outline connections between HuR, cancer, neuroinflammation and asthma.
About the Speaker:
Dr. Ulus Atasoy is currently Vice Chairman of Research in the Department of Surgery, Director of the MD-PhD Program at the University of Missouri, and Chief of Allergy-Immunology at the Harry S. Truman Veterans Hospital in Columbia, Missouri, USA. He attended graduate and medical school at the University of Minnesota (Minneapolis, Minnesota) and completed pediatrics residency training at UCLA School of Medicine (Los Angeles, California). He did postdoctoral training at the National Institute of Health in the laboratory of Dr. William Paul and completed clinical fellowship training in Dr. Rebecca Buckley’s division at Duke University Medical Center (Durham, North Carolina). He studied posttranscriptional gene regulation in the laboratory of Dr. Jack Keene, where he cloned murine HuR (elavl1) and participated in the development of RNA immunoprecipitation techniques (RIP-Chip and RIP-Seq). Currently, the Atasoy lab works on posttranscriptional gene regulation in cancer, autoimmunity and allergic airway inflammation. Recent findings in his lab have demonstrated the important role HuR plays in regulation of CD4+ T cell differentiation and IL-2 homeostasis.