Mike Taussig
Mike Taussig is pursuing translational activities within Babraham Bioscience Technologies Ltd. (BBT)
Cell free protein display and array systems
In ribosome display technology, proteins and genes are directly linked in very large libraries from which we select and evolve human antibodies. The linkage is in the form of stable complexes comprising the nascent protein, ribosome and mRNA; complexes are selected by ligand binding and genes can be sensitively recovered by RT-PCR.
We also make functional protein arrays using the protein in situ array or PISA method. The proteins, which are expressed in cell free systems, have a novel hexa-Histidine tag and are made on surfaces precoated with the immobilising agent Ni-NTA, to which they adhere immediately after translation.
This enables one to go in a single step from DNA to protein array without the need for protein handling or purification. PISA and ribosome display are being combined to demonstrate protein-protein interactions in vitro, particularly between domains of proteins involved in signal transduction pathways.
Patent applications have been made on these technologies, which are being developed commercially in the Institute’s spinout company www.discerna.co.uk
Another major area is protein structure determination, in collaboration with a number of crystallography groups (Brian Sutton, KCL; Enrico Stura, Gif sur Yvette; Ian Wilson, Scripps Research Institute). Recently we have focused on human rheumatoid factors (autoantibodies prevalent in rheumatoid arthritis) and their complexes with IgG and bacterial Ig-binding proteins (Staphylococcus aureusprotein A, Peptostreptococcus magnusprotein L). We have also solved the structure of human placental alkaline phosphatase (with Torgny Stigbrand, Umea, Sweden).
Mike Taussig is Chairman of the European Science Foundation programme on Integrated Approaches to Functional Genomics, which is supported by research councils in 23 countries. Its aim is to promote the development of functional genomics in Europe through activities such as workshops, training courses, a large scale Euroconference and an informative website www.functionalgenomics.org.uk
Recent, selected publications
Duquerroy S, Stura EA, Bressanelli S, Fabiane SM, Vaney MC, Beale D, Hamon M, Casali P, Rey FA, Sutton BJ, Taussig MJ (2007) Crystal structure of a human autoimmune complex between IgM rheumatoid factor RF61 and IgG1 Fc reveals a novel epitope and evidence for affinity maturation.
Journal of Molecular Biology 368 1321-1331
http://dx.doi.org/10.1016/j.jmb.2007.02.085
He M, Taussig MJ (2007) Eukaryotic ribosmome display with in situ DNA recovery.
Nature Methods 4 281-288
http://dx.doi.org/10.1038/nmeth1001
Taussig MJ, Stoevesandt O, Borrebaeck CAK, Bradbury AR, Cahill D, Cambillau C, de Daruvar A, Dubel S, Eichler J, Frank R, Gibson TJ, Gloriam D, Gold L, Herberg FW, Hermjakob H, Hoheisel JD, Joos TO, Kallioniemi O, Koegll M, Konthur Z, Korn B, Kremmer E, Krobitsch S, Landegren U, van der Maarel S, McCafferty J, Muyldermans S, Nygren P-A, Palcy S, Pluckthun A, Polic B, Przybylski M, Saviranta P, Sawyer A, Sherman DJ, Skerra A, Templin M, Ueffing M, Uhlen M (2007) ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome.
Nature Methods 4 13-17
http://dx.doi.org/10.1038/nmeth0107-13
Khan F, He M, Taussig MJ (2006) Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on Ni-nitrilotriacetic acid surfaces
Analytical Chemistry 78 3072-3079
http://dx.doi.org/10.1021/ac0601841
He M, Taussig MJ (2005) Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system.
Journal of Immunological Methods 297 73-82
http://dx.doi.org/10.1016/j.jim.2004.11.022
Gullberg M, Fredriksson S, Taussig MJ, Jarvius J, Gustafsdottir S, Landegren U (2003) A sense of closeness: protein detection by proximity ligation.
Current Opinion in Biotechnology 14 82-86
http://dx.doi.org/10.1016/S0958-1669(02)00011-3
Graille M, Stura EA, Housden NG, Beckingham JA, Bottomley SP, Beale D, Taussig MJ, Sutton BJ, Gore MG, Charbonnier J-B (2001) Complex between Peptostreptococcus magnus protein L and a human antibody reveals structural convergence in the interaction modes of Fab binding proteins.
Structure 9 679-687
http://dx.doi.org/10.1016/S0969-2126(01)00630-X
He M, Taussig MJ (2001) Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method).
Nucleic Acids Research 29 e73 (Online only)
http://dx.doi.org/10.1093/nar/29.15.e73
Le Du MH, Stigbrand T, Taussig MJ, Menez A, Stura EA (2001) Crystal structure of alkaline phosphatase from human placenta at 1.8 [A angstrom] resolution: implication for a substrate specificity.
Journal of Biological Chemistry 276 9158-9165
http://dx.doi.org/10.1074/jbc.M009250200
Sutton BJ, Corper AL, Bonagura VR, Taussig MJ (2000) The structure and origin of rheumatoid factors.
Immunology Today 21 177-183
http://dx.doi.org/10.1016/S0167-5699(00)01589-9
Graille M, Stura EA, Corper AL, Sutton BJ, Taussig MJ, Charbonnier J-B, Silverman GJ (2000) Crystal structure of a Staphylococcus aureus protein A domain complexed with the Fab fragment of a human IgM antibody: structural basis for recognition of B-cell receptors and superantigen activity.
Proceedings of the National Academy of Sciences of the United States of America 97 5399-5404
http://www.pnas.org/cgi/content/full/97/10/5399
