Imaging

The Babraham Institute's Imaging Facility was established to meet a growing demand for fluorescence imaging across the campus. The Imaging Facility now offers access to a wide range of state-of-the-art imaging technologies including sensitive wide-field epi-fluorescence (with the option of deconvolution), high resolution point-scanning confocal, spinning disk confocal, Total Internal Reflection Fluorescence and High Content Screening. For image analysis and presentation the Facility has a suite of sophisticated image processing software.

For occasional users the Imaging Facility provides fully assisted support, but researchers are generally encouraged to use the equipment themselves following appropriate training and supervision. Training and support for work undertaken in the Facility is provided by Dr Simon Walker.

Imaging Facility Hardware

Point-scanning confocals: Zeiss LSM 510 META & Olympus FV1000
Spinning-disk confocal: PerkinElmer LCI
Wide-field epi-fluorescence: Olympus CellR
Total Internal Reflection Fluorescence (TIRF): Olympus CellR
High Content Screening (HCS): BD Pathway 855
Fluorescence spectrometer: PerkinElmer LS 55

Image Processing Software

Volocity (Improvision)
Autodeblur (Autoquant)

Images from top to bottom:

Cell type: Drosophila eye
Authors: Simon Walker, Raghu Padinjat

Cell type: HeLa
Author:Llewelyn Roderick

Cell type: Rat hippocampal neurons
Authors: Paul Cuddon, Simon Walker, Llewelyn Roderick, Martin Bootman