The Babraham Institute receives strategic funding from the BBSRC
The Babraham Institute receives strategic funding from the BBSRC
Scientific Services
Barrier Unit
Bioinformatics
FACS
Gene Targeting
Imaging
Lipidomics
Mass Spectrometry
Monoclonal Antibodies
Specialist Equipment
Corporate Services
Conference Facilities
Our gene targeting service is available to both institute scientists, and also on a fee-for-service basis to the general scientific community. The facility has over 10 years experience and offers best practices and methods at the cutting edge of this technically challenging discipline.
Transgenics can be produced by pronuclear injection of plasmid DNA or, by BAC injection. We can generate knock-out and knock-in along with conditional knockouts both in vitro and in vivo with high success rates.
A variety of embryonic stem cell lines are available for use, from the conventional mouse 129 Ola line to the C57Bl/6 line which has recently been validated; this latest embryonic stem cell line is of enormous value in terms of efficiency.
• Generation of ES cell clones containing constitutive or conditional targeted mutations
• Transient recombinase-mediated deletion of DNA sequences in ES clones (Subject to Licence)
• Blastocyst injection of positive clones for chimera development
• DNA pro-nuclear microinjection leading to founder offspring.
Babetto, E. et al (2010) Targeting NMNAT1 to axons and synapses transforms its neuroprotective potency
J Neurosci 30: 13291-13304 (PubMed)
Beirowski, B. et al (2010) WldS can delay Wallerian degeneration in mice when interaction with valosin containing protein is weakened.
Neuroscience 166: 201-11. (PubMed)
Hodson, D. J. et al (2010) Deletion of the RNA-binding proteins zfp36L1 and zfp36L2 leads to perturbed thymic development and T-lymphoblastic leukaemia.
Nat Immunol 11(8):717-724 (PubMed)
Ramadani, F. et al (2010) The PI3K isoforms p110α and p110δ are essential for pre-B cell receptor signaling and B cell development.
Sci Signal 3(134):ra60 (PubMed)
Rolf, J. et al (2010) Phosphoinositide 3-Kinase Activity in T Cells Regulates the Magnitude of the Germinal Center Reaction.
J Immunol 185: 4042-4052 (PubMed)
Saunders,A. et al (2010) Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes.
Blood 115:3249-57 (PubMed)
Beirowski, B. et al (2009) Non-nuclear WldS determines its neuroprotective efficacy for axons and synapses in vivo
J Neurosci 29: 653-68 (PubMed)
Chotalia, M. et al (2009) Transcription is required for establishment of germline methylation marks at imprinted genes.
Genes & Dev 23: 105-117 (PubMed)
Conforti, L. et al (2009) WldS protein requires Nmnat activity and a short N-terminal sequence to protect axons in mice.
J Cell Biol 184: 491-500 (PubMed)
Graupera, M. et al (2008) Angiogenesis selectively requires the p110α isoform of PI3K to control endothelial cell migration. Nature 453(7195):662-6 (PubMed)
Guillermet-Guibert, J. et al (2008) The p110β isoform of phosphoinositide 3-kinase signals downstream of G protein-coupled receptors and is functionally redundant with p110γ.
Proc Natl Acad Sci U S A. 105(24):8292-7 (PubMed)
Babraham Institute - Babraham Research Campus - Cambridge - United Kingdom